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portada Calcium Entry Blockers in Cardiovascular and Cerebral Dysfunctions
Type
Physical Book
Publisher
Author
Language
English
Pages
325
Format
Paperback
ISBN13
9789400960350
Categories

Calcium Entry Blockers in Cardiovascular and Cerebral Dysfunctions

Godfraind, T. ; Herman, A. G. ; Wellens, D. (Author) · Springer · Paperback

Calcium Entry Blockers in Cardiovascular and Cerebral Dysfunctions - Godfraind, T. ; Herman, A. G. ; Wellens, D.

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Synopsis "Calcium Entry Blockers in Cardiovascular and Cerebral Dysfunctions"

2 The free internal Ca+ concentration in human red cells is set according to the leak- 2 and-pump principle: There is a finite passive Ca+ influx at the physiological 2 2 Ca+ -gradient across the membrane which is compensated by Ca+ pumping in the outward direction with a rate given by the degree of saturation of the A TP-fuelled Ca- 2 pump at the steady-state internal Ca+ concentration. Simons (1982) recently devised a method allowing the measurement of the steady- 2 2 state internal Ca+ concentration. Cells are suspended in media of different Ca+ con- 2 2 tent whose Ca+ concentration is monitored by a Ca+ -selective electrode. When the cells are lysed (by digitonin) there is an upward or downward deflection of the elec- 2 trode signal. At the point of zero deflection, the cellular Ca+ concentration equals that 2 of the medium. The result is, that in fresh human red blood cells the Ca+ concentra- tion is;;;; O.4, uM (this is an upper estimate; the true value may be considerably lower).

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